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Presepsin (sCD14-ST) secretion and kinetics by peripheral blood mononuclear cells and monocytic THP-1 cell line
Camille Chenevier-Gobeaux1, Valérie Bardet2, Hélène Poupet3
1Service de diagnostic biologique automatisé, Groupe hospitalier Broca Cochin Hôtel-Dieu, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France.
Abstract:
Presepsin could help for early diagnosis of systemic infection. Little is known regarding its kinetics. We studied presepsin concentration after challenge with bacterial agonist lipopolysaccharide (LPS) stimulation in peripheral mononuclear cells (PMNC) collected from 5 healthy volunteers and in a human cell line of monocytic cells (THP1). In PMNC, an exposure to LPS (100 ng/mL) induced an increase of median presepsin levels as early as hour 1 (+31%, p=0.007), concomitantly to IL-6 synthesis. In THP1 cells, presepsin was detected at 1 hour after LPS exposure, and peaked at 3 hours, in THP1 cells. In conclusion, we report here that presepsin, a surrogate marker of the host response to bacteria, increases early in PMNC and in a monocytic cell lineage. Our findings might confirm the potential usefulness of presepsin bedside as an early marker of infectious diseases.
Insights
Presepsin levels rise quickly after bacterial stimulation in immune cells and cell lines. This early increase suggests presepsin
Area of Science:
- Biochemistry
- Immunology
- Cell Biology
Background:
- Presepsin is a potential biomarker for early systemic infection diagnosis.
- Limited data exists on presepsin kinetics following bacterial stimulation.
Purpose of the Study:
- To investigate the early kinetics of presepsin expression.
- To evaluate presepsin response to bacterial lipopolysaccharide (LPS) stimulation in human cells.
Main Methods:
- Peripheral mononuclear cells (PMNC) from healthy volunteers and THP1 monocytic cells were stimulated with LPS (100 ng/mL).
- Presepsin and IL-6 concentrations were measured over time.
- Kinetic analysis of presepsin expression was performed.
Main Results:
- In PMNC, LPS induced a significant increase in presepsin levels by 1 hour (+31%, p=0.007), coinciding with IL-6 synthesis.
- In THP1 cells, presepsin was detectable at 1 hour and peaked at 3 hours post-LPS exposure.
- These findings demonstrate an early presepsin response in both primary immune cells and a monocytic cell line.
Conclusions:
- Presepsin, a marker of host response to bacteria, shows early kinetics in peripheral mononuclear cells and monocytic cell lines.
- The rapid increase in presepsin following bacterial challenge supports its potential utility as an early diagnostic marker for infectious diseases at the bedside.
