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Paenibacillus larvae Phage Tripp Genome Has 378-Base-Pair Terminal Repeats
J Abraham1, A-C Bousquet1, E Bruff1
1Department of Plant & Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA.
Genome Announcements
|January 9, 2016
Summary
A novel bacteriophage, named Tripp, was discovered in honey bees with American foulbrood disease. This unique phage possesses a distinct genome, offering new insights into bee pathogen research.
Area of Science:
- Microbiology
- Virology
- Apiculture
Background:
- American foulbrood is a destructive bacterial disease affecting honey bee larvae.
- Paenibacillus larvae is the causative agent of American foulbrood.
- Bacteriophages are viruses that infect bacteria and can be explored as potential biocontrol agents.
Purpose of the Study:
- To isolate and characterize a novel bacteriophage targeting Paenibacillus larvae.
- To analyze the genomic features of the isolated bacteriophage.
Main Methods:
- Isolation of bacteriophage from diseased honey bee hive samples.
- Genome sequencing and analysis of the isolated bacteriophage.
- Bioinformatic analysis to identify encoded proteins and genomic features.
Main Results:
- Paenibacillus larvae bacteriophage Tripp was successfully isolated from a North Carolina apiary.
- The complete genome sequence of bacteriophage Tripp is 54,439 base pairs with a G+C content of 48.3%.
- The genome encodes 92 proteins, lacks tRNA genes, and features 378-bp direct terminal repeats, representing a unique sequence in GenBank.
Conclusions:
- Bacteriophage Tripp represents a novel viral entity with potential applications in managing American foulbrood.
- The unique genomic characteristics of Tripp warrant further investigation for its biological significance and potential use in bee health strategies.
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