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Updated: Mar 27, 2026
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
Published on: January 4, 2016
Acetate selective fluorescent turn-on sensors derived using vitamin B6 cofactor pyridoxal-5-phosphate
Darshna Sharma1, Aman Kuba1, Rini Thomas1
1Department of Applied Chemistry, SV National Institute Technology, Surat, Gujarat, India.
Abstract:
Two new Schiff base receptors have been synthesized by condensation of pyridoxal-5-phosphate with 2-aminophenol (L(1)) or aniline (L(2)). In DMSO, the receptors showed both chromogenic and 'turn-on' fluorescence responses selectively in the presence of AcO(-) and F(-). However, in mixed DMSO-H2O medium, the receptors showed AcO(-) selective 'turn-on' fluorescence without any interference from other tested anions including F(-). The detection limit for AcO(-) was found to be 7.37 μM and 22.9 μM using the receptors L(1) and L(2), respectively.
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