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A problem encountered using staphylococcus/streptococcus supplement.

C E Morton, H A Holt

    Medical Laboratory Sciences
    |January 1, 1989
    PubMed
    Summary
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    A commercial supplement for blood agar culture media inhibits Staphylococcus aureus growth. This occurs due to a pH change in carbon dioxide-enriched atmospheres, potentiating nalidixic acid. Avoid incubating media with this supplement in CO2.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics

    Background:

    • Selective culture media are crucial for isolating specific microorganisms.
    • Commercial supplements aim to enhance selectivity and growth of target bacteria.

    Purpose of the Study:

    • To investigate the cause of poor Staphylococcus aureus growth on selective blood agar.
    • To identify factors affecting the efficacy of a staphylococcus/streptococcus selective supplement.

    Main Methods:

    • Comparative growth studies of Staphylococcus aureus on selective and non-selective blood agar media.
    • Analysis of environmental factors, specifically carbon dioxide (CO2) atmosphere, on bacterial growth.
    • Assessment of pH changes in the culture medium.

    Main Results:

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  • Staphylococcus aureus strains exhibited poor growth on selective blood agar compared to non-selective media.
  • Incubation in a CO2-enriched atmosphere caused a pH shift in the selective medium.
  • This pH change potentiated the inhibitory activity of nalidixic acid within the supplement.

    • Conclusions:

    • The commercial staphylococcus/streptococcus selective supplement's efficacy is compromised in CO2-enriched atmospheres.
    • Nalidixic acid activity is enhanced by pH changes induced by CO2 incubation, inhibiting Staphylococcus aureus.
    • It is recommended to avoid incubating blood agar media containing this supplement in CO2-enriched environments.