Trypsin digest of the iris
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A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
Published on: June 22, 2011
1Department of Ophthalmology, University of Texas Medical Branch, Galveston.
Ophthalmic Research
|January 1, 1989
Abstract:
We succeeded in obtaining vascular mounts of the iris by modifying the original technique of trypsin digestion described by Kuwabara and Cogan. This was achieved by scraping the pigment epithelium, repeated bleaching, and prolonged enzymatic digestion.
