Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

29
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
29
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

78
Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
78
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

46
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
46
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

31
Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
31
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

873
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
873
Antimicrobial Proteins01:23

Antimicrobial Proteins

15.4K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
15.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cystobactamid off-target profiling reveals favorable safety, superoxide reduction, and SCARB1 inhibition in eukaryotes.

npj drug discovery·2026
Same author

Centrifugal microfluidic automation of the protein aggregation capture workflow for robust mass spectrometry-based proteomics.

Lab on a chip·2026
Same author

Natural Product-Derived Ianthelliformisamines Inhibit Protein Translation and Block Bacterial Flagellum Assembly.

ACS chemical biology·2026
Same author

Bioresponsive pseudoGlucosinolates (psGSLs) Release Isothiocyanates (ITCs) in the Presence of Nitroreductases.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Chemical Proteomics Reveal the Inventory of Pyrroloquinoline Quinone Binding Proteins in Bacteria.

Journal of the American Chemical Society·2026
Same author

Metronidazole and ether derivatives target Helicobacter pylori via simultaneous stress induction and inhibition.

Nature microbiology·2026
Same journal

A Selectfluor-based Polonovski Rearrangement Leading to Novel Entities for Synthetic and Medicinal Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Relay Approach: A Convergent Synthesis of Key Fragments en route to (+)-Neosorangicin A.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Working Under Pressure: Empirical Findings on the Challenges Facing PhD Students in Chemistry.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Charge Resonance Interaction in Aromatic Trimer Radical Cations Revealed by IR Spectroscopy: The Case of Pyrrole Homo- and Heterotrimers.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

5'-Carboxy-Nucleoside Decarboxylation for the Synthesis of C-Nucleoside-Amino Acids and Peptides.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Synthesis, Characterization, and Redox Reactions at the Solid-Liquid Interface of Submicron-Size Ru(edta) Complex.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: Mar 27, 2026

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
06:36

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds

Published on: September 8, 2021

3.3K

An Aromatic Hydroxyamide Attenuates Multiresistant Staphylococcus aureus Toxin Expression.

Jan Vomacka1, Vadim S Korotkov1, Bianca Bauer1

  • 1Department of Chemistry, AVIRU, GO-Bio-project of the Federal Ministry of Education and Research, FKZ: 031A131, Technische Universität München (TUM), Lichtenbergstrasse 4, 85747, Garching, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 11, 2016
PubMed
Summary
This summary is machine-generated.

A novel hydroxyamide compound effectively reduces toxins in Methicillin-resistant Staphylococcus aureus (MRSA) infections. This approach targets virulence, offering a promising alternative to traditional antibiotics and potentially overcoming resistance.

Keywords:
click chemistrydrug discoverymedicinal chemistryproteomicstoxicology

More Related Videos

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
12:27

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Published on: January 3, 2020

6.9K
A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.7K

Related Experiment Videos

Last Updated: Mar 27, 2026

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
06:36

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds

Published on: September 8, 2021

3.3K
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
12:27

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Published on: January 3, 2020

6.9K
A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.7K

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Drug Discovery

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) infections pose a significant global health threat due to limited effective treatments.
  • The emergence of antibiotic resistance necessitates the development of novel therapeutic strategies with unique mechanisms of action.

Purpose of the Study:

  • To identify and characterize a new hydroxyamide compound with anti-virulence properties against S. aureus and MRSA.
  • To elucidate the molecular mechanisms underlying the compound's efficacy.
  • To evaluate the therapeutic potential of the hydroxyamide in a preclinical MRSA infection model.

Main Methods:

  • Transcriptome analysis to assess gene expression changes.
  • Affinity-based protein profiling and mass spectrometry-based chemical proteomics to identify molecular targets.
  • In vitro assays to evaluate resistance development.
  • In vivo efficacy studies using a MRSA mouse skin infection model.

Main Results:

  • The hydroxyamide compound significantly reduced the expression of key virulence factors and toxins in S. aureus and MRSA strains.
  • Transcriptome analysis indicated the inhibition of a central virulence pathway.
  • Chemical proteomics identified putative molecular targets for the compound.
  • Systemic treatment with the hydroxyamide led to a significant reduction in abscess size in a mouse model.
  • No resistance to the compound developed in vitro.

Conclusions:

  • Hydroxyamide compounds represent a promising new class of anti-virulence agents against MRSA.
  • Targeting bacterial virulence, rather than direct killing, offers a strategy to circumvent resistance and prolong therapeutic effectiveness.
  • This approach holds potential for developing novel treatments for challenging S. aureus and MRSA infections.