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FKBP8 interact with classical swine fever virus NS5A protein and promote virus RNA replication

Helin Li1, Chengcheng Zhang1, Hongjie Cui1

  • 1College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.

Virus Genes
|January 11, 2016
PubMed

Insights

Classical swine fever virus (CSFV) non-structural protein 5A (NS5A) interacts with FKBP8, promoting viral RNA synthesis. FKBP8 is crucial for CSFV replication, offering potential therapeutic targets.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Classical swine fever virus (CSFV) non-structural protein 5A (NS5A) is implicated in viral replication and modulation of host responses.
  • FKBP8 is a cellular protein known to support viral replication.

Purpose of the Study:

  • To investigate the interaction between CSFV NS5A and FKBP8.
  • To elucidate the role of FKBP8 in CSFV replication.

Main Methods:

  • Coimmunoprecipitation and GST-pulldown assays to confirm NS5A-FKBP8 interaction.
  • Confocal microscopy to visualize subcellular localization of NS5A and FKBP8.
  • Functional assays involving FKBP8 overexpression and knockdown (using lentivirus-mediated shRNA) to assess impact on viral RNA synthesis and replication.

Main Results:

  • Specific interaction between CSFV NS5A and FKBP8 was demonstrated.
  • NS5A and FKBP8 were found to colocalize in the cytoplasm.
  • FKBP8 overexpression significantly enhanced CSFV RNA synthesis, while FKBP8 knockdown markedly reduced viral replication.

Conclusions:

  • FKBP8 plays a critical role in the CSFV life cycle, particularly in viral RNA replication.
  • The interaction between NS5A and FKBP8 is essential for efficient CSFV replication.
  • Targeting FKBP8 function presents a potential strategy for developing novel treatments against CSFV infection.
Keywords:
Classical swine fever virus (CSFV)FKBP8InteractionNS5A

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