Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pyroxd1 is essential for murine viability with the homozygous N155S recurrent variant linked to myopathy, muscle hypotrophy and osteopenia.

Acta neuropathologica communications·2026

Same author

Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development.

Nature communications·2026

Same author

Integrated multi-omic atlas reveals the hierarchy of spatiotemporal regulatory networks of mouse gastrulation.

Nature communications·2026

Same author

Constructing the spatiotemporal atlas of single-cell lineage trajectories in stereotypic biological structures.

iScience·2026

Same author

Procollagen IIA mediates positive feedback control of the mouse cardiogenic transcriptional network.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells.

Stem cell reports·2025

Search research articles

Related Experiment Video

Updated: Mar 27, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Human Stem Cells Can Differentiate in Post-implantation Mouse Embryos.

Patrick P L Tam¹

¹Embryology Unit, Children's Medical Research Institute, Sydney Medical School, University of Sydney, 214 Hawkesbury Road, Westmead, NSW 2145, Australia.

|January 11, 2016

Summary

Human pluripotent stem cells (hPSCs) differentiation potential can be assessed using a novel human-mouse chimera model. This ex vivo approach in mouse embryos provides an efficient alternative to traditional methods for evaluating germ layer development.

More Related Videos

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

Related Experiment Videos

Last Updated: Mar 27, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

Area of Science:

Developmental biology
Stem cell research
Regenerative medicine

Background:

Assessing human pluripotent stem cells (hPSCs) differentiation capacity is crucial for regenerative medicine.
Conventional methods like teratoma induction and in vitro assays have limitations.

Purpose of the Study:

To introduce and validate a new method for evaluating the germ layer differentiation potential of hPSCs.
To compare the efficiency of the new method against traditional assays.

Main Methods:

Utilizing a human-mouse post-implantation chimera model.
Culturing human pluripotent stem cells (hPSCs) within mouse embryos ex vivo.
Analyzing germ layer derivative formation from hPSCs.

Main Results:

The human-mouse chimera model efficiently demonstrates the germ layer differentiation potential of hPSCs.
This ex vivo approach offers a viable alternative to in vivo teratoma assays.

Conclusions:

The human-mouse chimera model is an effective tool for assessing hPSC pluripotency and differentiation capacity.
This method advances the study of early human development and stem cell biology.