Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Helodermin stimulates glucagon secretion in the mouse.

B Ahrén1

  • 1Department of Pharmacology, Lund University, Sweden.

Peptides
|May 1, 1989
PubMed
Summary

Helodermin significantly boosts glucagon secretion in mice, similar to vasoactive intestinal peptide (VIP). This effect is partially mediated by alpha- and beta-adrenoceptors, influencing glucose regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impaired glucose tolerance (IGT) is associated with reduced insulin-induced suppression of glucagon concentrations.

Diabetologia·2001
Same author

The expression of hormone-sensitive lipase in clonal beta-cells and rat islets is induced by long-term exposure to high glucose.

Diabetes·2001
Same author

FOXC2 is a winged helix gene that counteracts obesity, hypertriglyceridemia, and diet-induced insulin resistance.

Cell·2001
Same author

Contribution to glucose tolerance of insulin-independent vs. insulin-dependent mechanisms in mice.

American journal of physiology. Endocrinology and metabolism·2001
Same author

The neuropeptide pituitary adenylate cyclase-activating polypeptide and islet function.

Diabetes·2001
Same author

Impaired incretin response after a mixed meal is associated with insulin resistance in nondiabetic men.

Diabetes care·2001

Area of Science:

  • Endocrinology
  • Neuroendocrinology
  • Gastrointestinal Physiology

Background:

  • Helodermin shares structural similarities with vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI).
  • VIP and PHI are known to stimulate both insulin and glucagon secretion.
  • The specific effects of helodermin on pancreatic hormone secretion require elucidation.

Purpose of the Study:

  • To investigate the impact of helodermin on insulin and glucagon secretion in mice.
  • To examine helodermin's effects under basal conditions and during glucose and carbachol stimulation.
  • To explore the role of adrenoceptors in helodermin-induced glucagon secretion.

Main Methods:

  • Intravenous administration of helodermin to mice at varying dose levels (0.5–8.0 nmol/kg).
  • Measurement of plasma glucagon and insulin levels under basal conditions.
  • Assessment of helodermin's effects in conjunction with glucose or the cholinergic agonist carbachol.
  • Evaluation of helodermin's action during combined alpha- and beta-adrenoceptor blockade (yohimbine + L-propranolol).

Main Results:

  • Helodermin markedly enhanced basal plasma glucagon levels without affecting basal insulin.
  • Helodermin augmented plasma glucagon levels when co-administered with glucose, but did not alter insulin levels.
  • Helodermin significantly potentiated carbachol-induced glucagon secretion, with no effect on insulin.
  • Combined alpha- and beta-adrenoceptor blockade reduced helodermin's augmenting effect on glucagon secretion by approximately 60%.

Conclusions:

  • Helodermin is a potent stimulator of glucagon secretion in mice.
  • The stimulatory effect of helodermin on glucagon secretion is partially mediated by alpha- and beta-adrenoceptors.
  • Helodermin's action on glucagon secretion is independent of insulin secretion under the tested conditions.

Related Experiment Videos