Manipulation of avian primordial germ cells and gonadal differentiation

The authors hypothesized that donor primordial germ cells (PGC) are useful as vehicles of gene transfer in birds. The PGC have been identified in the blastula prior to incubation. They have been isolated from the Stage 7 germinal crescents, Stage 17 blood, and Stage 30 gonads. The percentages of PGC in these three embryonic sources were 2, .003 and 1.5%, respectively. The isolated PGC have been used as antigens to produce specific antibodies that have served as major tools in identification, isolation, and forming enriched in vitro cultures of PGC. Sterile hosts for normal donor PGC have been induced by use of ultraviolet irradiation, treatment with the chemical busulfan, and crossing rooster with female quail to form a sterile host termed "quickens." Micromanipulator technology, along with refinement of techniques to avoid trauma, will allow 80% hatchability. Integration of all the above biotechnology has led to successful use of PGC as vehicles of gene transfer and production of autogenic quail.