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Ratiometric Fluorescent Pattern for Sensing Proteins Using Aqueous Polymer-Pyrene/γ-Cyclodextrin Inclusion Complexes.

Nan Li1,2, Li Qi1, Juan Qiao1

  • 1Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.

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A novel fluorescent sensor distinguishes proteins using polymer-pyrene/γ-cyclodextrin inclusion complex dissociation. This method offers tunable selectivity and sensitivity for protein sensing applications.

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Area of Science:

  • Supramolecular Chemistry
  • Analytical Chemistry
  • Polymer Chemistry

Background:

  • Fluorescent sensors are crucial for detecting biomolecules.
  • Distinguishing between different protein types remains a challenge.
  • Inclusion complexes offer unique platforms for molecular recognition.

Purpose of the Study:

  • To develop a ratiometric fluorescent sensor for protein detection and differentiation.
  • To utilize the dissociation of polymer-pyrene/γ-cyclodextrin inclusion complexes for sensing.
  • To achieve tunable selectivity and sensitivity in protein sensing.

Main Methods:

  • Synthesis of aqueous polymer-pyrene via atom transfer radical polymerization.
  • Formation of polymer-pyrene/γ-cyclodextrin inclusion complexes exhibiting excimer emission.
  • Detection of nonmetalloproteins via inclusion complex dissociation and ratiometric fluorescence changes.
  • Detection of metalloproteins via fluorescence quenching due to energy transfer.

Main Results:

  • The developed inclusion complexes showed distinct responses to different proteins.
  • Nonmetalloproteins induced dissociation, altering pyrene excimer/monomer emission.
  • Metalloproteins quenched pyrene fluorescence via energy transfer.
  • Sensor selectivity and sensitivity were tunable by altering polymer type and chain length.

Conclusions:

  • A versatile and selective fluorescent platform for protein sensing was established.
  • The ratiometric sensing mechanism allows for reliable protein detection.
  • The tunable nature of the sensor holds promise for various protein analysis applications.