Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chloroquine and acid vesicle function.

D J Krogstad1, P H Schlesinger, I Y Gluzman

  • 1Department of Medicine, Washington University School of Medicine, St. Louis, MO.

Progress in Clinical and Biological Research
|January 1, 1989
PubMed
Summary

Chloroquine works by raising the pH of the malaria parasite's vesicles. Chloroquine resistance in Plasmodium falciparum is caused by the rapid efflux of the drug from the parasite.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of seasonality and ecological factors on the prevalence of the four malaria parasite species in northern mali.

Journal of tropical medicine·2012
Same author

Calcium signalling and calcium transport in bone disease.

Sub-cellular biochemistry·2008
Same author

The Bax pore in liposomes, Biophysics.

Cell death and differentiation·2006
Same author

The Jordan family legacy: three generations of dentistry.

Texas dental journal·2002
Same author

Genetic toxicology testing of the antimalarial drugs chloroquine and a new analog, AQ-13.

Environmental and molecular mutagenesis·2001
Same author

Placental changes associated with fetal outcome in the Plasmodium coatneyi/rhesus monkey model of malaria in pregnancy.

The American journal of tropical medicine and hygiene·2001

Area of Science:

  • Malariology
  • Parasitology
  • Pharmacology

Background:

  • Chloroquine is a key antimalarial drug.
  • Understanding its mechanism and resistance is crucial for malaria treatment.

Purpose of the Study:

  • To investigate the site and mechanism of chloroquine action in malaria parasites.
  • To elucidate the basis of chloroquine resistance in Plasmodium falciparum.

Main Methods:

  • Measuring parasite vesicle pH.
  • Assessing 3H-chloroquine accumulation in parasite vesicles.
  • Analyzing chloroquine efflux in resistant strains.

Main Results:

  • Chloroquine acts within the parasite's acid intracellular vesicle system.
  • The initial step involves increasing parasite vesicle pH.
  • Parasites possess a chloroquine-concentrating mechanism in vesicles.
  • Chloroquine resistance is attributed to rapid drug efflux from the parasite.

Conclusions:

  • The acid vesicle system is the primary site of chloroquine action.
  • Elevating vesicle pH is essential for chloroquine's antimalarial effect.
  • Rapid efflux of chloroquine explains resistance in Plasmodium falciparum.

Related Experiment Videos