Single-stranded circular DNA generated from broad host range plasmid R1162 and its derivatives
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M Kok1, A C Arnberg, B Witholt
1Groningen Biotechnology Center, The Netherlands.
Plasmid
|May 1, 1989
Abstract:
Extraction of R1162 plasmid DNA with the alkaline lysis method yields considerable amounts of single-stranded circular plasmid DNA. Destabilization of plasmid DNA is stimulated by the R1162 mob region in cis. The formation of single-stranded circular DNA is initiated at a specific site on the plasmid, presumably the origin of transfer (oriT).