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Updated: Mar 27, 2026

Reusable Single Cell for Iterative Epigenomic Analyses
Published on: February 11, 2022
Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
Christof Angermueller1, Stephen J Clark2, Heather J Lee2,3
1European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
None:
We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
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