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Related Experiment Video

Updated: Mar 27, 2026

Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms
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Versatile size-dependent sorting of C. elegans nematodes and embryos using a tunable microfluidic filter structure.

Li Dong1, Matteo Cornaglia1, Thomas Lehnert1

  • 1Laboratory of Microsystems, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. martin.gijs@epfl.ch.

Lab on a Chip
|January 13, 2016
PubMed
Summary

Researchers developed a microfluidic device to sort Caenorhabditis elegans (C. elegans) by size. This method efficiently separates different larval stages and embryos, enabling synchronized populations for biological research and drug screening.

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Area of Science:

  • Developmental Biology
  • Microfluidics
  • Model Organisms

Background:

  • Caenorhabditis elegans (C. elegans) is a vital model organism for studying aging and disease.
  • Synchronized C. elegans populations are crucial for accurate bio-assays, but obtaining them is challenging.
  • Existing methods for C. elegans synchronization are often laborious or lack precision.

Purpose of the Study:

  • To introduce a novel microfluidic device for size-dependent sorting of C. elegans.
  • To enable the generation of stage- or age-synchronized C. elegans populations for research.
  • To improve the efficiency and throughput of C. elegans sorting for bio-assays.

Main Methods:

  • A microfluidic chip utilizing pressure-deformable polydimethylsiloxane (PDMS) transfer channels was designed.
  • External pressure was applied to tune the effective cross-section of channels, creating adjustable filters.
  • Size-dependent sorting of C. elegans larvae and embryos was achieved by optimizing pressure settings.

Main Results:

  • The device achieved near 100% efficiency in extracting specific larval stages from mixed cultures.
  • A throughput of up to 3.5 worms per second was demonstrated.
  • The system successfully sorted embryos from adult worms, facilitating age-synchronized populations.

Conclusions:

  • This microfluidic approach offers a simple, tunable, and efficient method for C. elegans sorting.
  • The technology supports the generation of synchronized populations for various C. elegans-based assays.
  • The device has significant potential for integration into automated, high-throughput microfluidic platforms.