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Interleukin-6 immunoreactivity in human tumors.
S S Tabibzadeh1, D Poubouridis, L T May
1Department of Pathology, City Hospital Center, Elmhurst, NY 11373.
The American Journal of Pathology
|September 1, 1989
Summary
Interleukin-6 (IL-6) is a key cytokine involved in cancer. This study found IL-6 is present in most human tumors, supporting its role in host-tumor interactions.
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- Interleukin-6 (IL-6) is implicated in systemic cancer alterations like fever and inflammation.
- IL-6 may also influence local tumor behavior, including proliferation, motility, and cell adhesion.
Purpose of the Study:
- To investigate the distribution of IL-6 immunoreactivity in various human tumors.
- To assess the role of IL-6 in host-tumor interactions.
Main Methods:
- Utilized the avidin-biotin-complex (ABC) procedure for IL-6 detection.
- Employed a polyclonal rabbit antiserum against human IL-6 (rIL-6).
- Included control experiments with preimmune serum and preadsorbed antiserum.
Main Results:
- Strong-to-moderate IL-6 immunoreactivity detected in squamous cell carcinomas, adenocarcinomas (mammary, colonic, ovarian, endometrial), metastatic adenocarcinomas, leiomyosarcoma, and neurofibrosarcoma.
- Weak-to-moderate IL-6 immunostaining observed in Hodgkin's and non-Hodgkin's lymphomas.
- Most human tumors examined stained positively for IL-6.
Conclusions:
- IL-6 is widely expressed in various human malignancies.
- The findings support IL-6's significant role as a cytokine in host-tumor interactions.