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Aberrant Maspin mRNA Expression is Associated with Clinical Outcome in Patients with Pulmonary Adenocarcinoma
Mingjie Lu1, Jun Li1, Zebo Huang1
1Department of Medical Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
Background:
The aim of this study was to investigate the expression level of maspin mRNA in pulmonary adenocarcinoma and to clarify its clinical significance in prediction of prognosis.
Material/Methods:
RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue blocks of 30 pairs of pulmonary adenocarcinoma (AC) tissues and adjacent noncancerous tissues (ANT) and in another 81 AC tissues. Expression of maspin mRNA was tested by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and the potential relationship between maspin mRNA expression and clinic pathological features of AC patients was analyzed.
Results:
The expression of maspin mRNA was upregulated in AC samples compared with the ANT (p<0.001). Patients at advanced clinical stage (III) and patients with lymphatic metastasis showed higher maspin mRNA expression level than those in early-stage patients (I and II) (p=0.038) or with non-lymphatic metastasis (p=0.034). The Kaplan-Meier survival curves indicated that disease-free survival (DFS) was significantly worse in high maspin mRNA expression AC patients (p=0.007). Furthermore, multivariate analysis revealed that the expression of maspin mRNA was an independent prognostic marker for AC (p=0.040).
Conclusions:
Our study reveals that maspin mRNA was significantly up-regulated in tissues of AC patients. Maspin mRNA may be useful as a new marker of prognosis in AC.
Insights
Maspin mRNA is upregulated in pulmonary adenocarcinoma (AC). High maspin mRNA expression correlates with advanced stage, metastasis, and worse disease-free survival, indicating its potential as a prognostic marker for AC.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Pulmonary adenocarcinoma (AC) is a significant cause of cancer-related mortality.
- Identifying reliable prognostic markers is crucial for improving patient outcomes in AC.
Purpose of the Study:
- To investigate maspin mRNA expression levels in AC tissues.
- To determine the clinical significance of maspin mRNA in predicting AC prognosis.
Main Methods:
- Maspin mRNA expression was quantified using quantitative reverse-transcription polymerase chain reaction (qRT-PCR).
- RNA was extracted from 30 pairs of AC tissues and adjacent noncancerous tissues (ANT), plus an additional 81 AC tissues.
- Correlation analysis was performed between maspin mRNA expression and clinicopathological features.
Main Results:
- Maspin mRNA was significantly upregulated in AC tissues compared to ANT (p<0.001).
- Higher maspin mRNA expression was observed in advanced stage (III) and lymphatically metastatic AC patients.
- High maspin mRNA expression was associated with significantly worse disease-free survival (DFS) (p=0.007) and identified as an independent prognostic marker (p=0.040).
Conclusions:
- Maspin mRNA is significantly upregulated in AC tissues.
- Maspin mRNA expression serves as a potential novel prognostic biomarker for pulmonary adenocarcinoma.
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