Related Experiment Video
Updated: Mar 27, 2026

Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
An innovative platform for quick and flexible joining of assorted DNA fragments
Henrique Cestari De Paoli1, Gerald A Tuskan1, Xiaohan Yang1
1Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, 37831, USA.
Abstract:
Successful synthetic biology efforts rely on conceptual and experimental designs in combination with testing of multi-gene constructs. Despite recent progresses, several limitations still hinder the ability to flexibly assemble and collectively share different types of DNA segments. Here, we describe an advanced system for joining DNA fragments from a universal library that automatically maintains open reading frames (ORFs) and does not require linkers, adaptors, sequence homology, amplification or mutation (domestication) of fragments in order to work properly. This system, which is enhanced by a unique buffer formulation, provides unforeseen capabilities for testing, and sharing, complex multi-gene circuitry assembled from different DNA fragments.
Related Concept Videos
Homologous Recombination
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Fixing Double-strand Breaks
DNA Isolation
DNA Isolation
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

