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Postganglionic sympathetic fibers (except those supplying the sweat glands) releasing noradrenaline or norepinephrine are called noradrenergic or adrenergic neurons. Noradrenaline, dopamine, adrenaline, or epinephrine are collectively called "catecholamines" as they contain a catechol moiety and an amine side chain. The five stages of neurotransmitter release involve their synthesis, storage, release, reuptake and metabolism.
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Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
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Published on: November 17, 2017

Plasma catecholamines: laboratory aspects.

K Nyyssönen1, M T Parviainen

  • 1Department of Clinical Chemistry, University of Kuopio, Finland.

Critical Reviews in Clinical Laboratory Sciences
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

This review compares plasma catecholamine analysis methods for clinical labs. Radioenzymatic assays offer high sensitivity, while liquid chromatography is faster and cheaper, but both require careful technique to avoid errors.

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Area of Science:

  • Clinical Chemistry
  • Analytical Biochemistry

Background:

  • Plasma catecholamines play crucial roles in physiological processes.
  • Accurate measurement of catecholamines is vital for diagnosing various medical conditions.

Purpose of the Study:

  • To review and evaluate common methods for plasma catecholamine analysis in clinical settings.
  • To compare the advantages and limitations of different analytical techniques.

Main Methods:

  • Discussion of indirect radioenzymatic assays.
  • Evaluation of direct determination using high-performance liquid chromatography (HPLC) with electrochemical or fluorometric detection.
  • Brief mention of radioimmunoassay and gas-chromatography.

Main Results:

  • Radioenzymatic assays provide high sensitivity, requiring small sample volumes.
  • HPLC methods are less tedious, faster, cheaper, and avoid radionuclides.
  • Both methods are susceptible to analytical errors if not properly developed and executed.

Conclusions:

  • Proper method development and skilled execution are essential for accurate plasma catecholamine analysis.
  • Choice of method depends on balancing sensitivity, cost, speed, and technical expertise.
  • Routine use of radioimmunoassay and gas-chromatography for plasma catecholamines is limited.