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LAMA tumor in the rat as an experimental model for pre-B-cell leukemia
M W De Jonge1, L De Leij, G Mesander
1Department of Clinical Immunology, University of Groningen, The Netherlands.
Abstract:
A late pre-B-cell leukemia model in the rat, the LAMA tumor, is described. A mouse monoclonal antibody (HIS30) was developed against LAMA cells. HIS30 reacts with a membrane antigen in tumor tissue, whereas its reactivity with normal tissues is limited to the zona glomerulosa of the adrenal cortex and to the adrenal medulla. HIS30 was used for both the immunohistological detection of tumor cells in tissue sections and the immunolocalization of tumor cells in vivo. To enable in vitro studies with the LAMA model, an in vitro growing cell line (LAMA-K1) was established from the LAMA tumor. LAMA-K1 is immunophenotypically similar to the original tumor. Two tumor transplantation models were characterized. In the first model LAMA was implanted s.c., and local tumor growth occurred at the injection site, which was then followed by lymphatogenic and subsequently hematogenic tumor spread. In the second model i.v. transplantation caused direct hematogenic tumor dissemination. In both models early dissemination was especially prominent to the bone marrow, spleen, and liver. Later in the disease most visceral organs became involved, and partial paralysis of the animal was observed in the end stage of the disease. In combination with HIS30, the LAMA pre-B-cell tumor offers a model for both the investigation of in vivo transplanted tumor cells and for the in vivo detection of tumor cells by HIS30 in LAMA tumor-bearing rats.
Insights
A new rat model for late pre-B-cell leukemia (LAMA tumor) and a specific antibody (HIS30) were developed. This model aids in studying leukemia progression and in vivo tumor detection.
Area of Science:
- Oncology
- Immunology
- Animal Models
Background:
- The LAMA tumor is a late pre-B-cell leukemia model in rats.
- A mouse monoclonal antibody, HIS30, was generated against LAMA cells.
Purpose of the Study:
- To characterize the LAMA tumor as a pre-B-cell leukemia model.
- To evaluate the utility of the HIS30 antibody for tumor detection and localization.
- To establish an in vitro cell line (LAMA-K1) for further studies.
Main Methods:
- Development of a mouse monoclonal antibody (HIS30) against LAMA cells.
- Characterization of LAMA tumor cell antigen reactivity in normal and tumor tissues.
- Establishment of an in vitro growing cell line (LAMA-K1) from the LAMA tumor.
- Establishment and characterization of two in vivo tumor transplantation models (subcutaneous and intravenous).
Main Results:
- HIS30 reacts with a membrane antigen on LAMA cells, with limited reactivity in normal adrenal tissues.
- LAMA-K1 cells are immunophenotypically similar to the original LAMA tumor.
- Both transplantation models showed early dissemination to bone marrow, spleen, and liver, with later involvement of visceral organs.
- The LAMA model demonstrated tumor spread via lymphatogenic and hematogenic routes, leading to paralysis in late stages.
Conclusions:
- The LAMA tumor in rats serves as a valuable model for studying late pre-B-cell leukemia.
- The HIS30 antibody is effective for immunohistological detection and in vivo tumor cell localization in this model.
- The established LAMA-K1 cell line and transplantation models facilitate in vitro and in vivo investigations of leukemia progression and treatment.