Related Experiment Video
Updated: Jul 11, 2026

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Published on: August 31, 2014
HIV-1 Gag mutants can dominantly interfere with the replication of the wild-type virus
D Trono1, M B Feinberg, D Baltimore
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Abstract:
The products of the human immunodeficiency virus (HIV) gag gene exist in a highly multimerized state in the mature virion. For that reason, they may represent a particularly suitable target for the generation of dominant negative mutants. A number of HIV site-directed Gag mutants did show interference with the production of infectious viral particles from cells in which they were cotransfected with a wild-type proviral DNA. Furthermore, cells constitutively expressing such HIV Gag mutants had an impaired ability to support HIV replication when infected with wild-type virus. The block was localized to the late stages of the virus life cycle. Such Gag variants could constitute prototypes for the development of anti-HIV intracellular immunization.
Related Concept Videos
Retrovirus Life Cycles
Viral Mutations
Retroviruses
Size and Structure of Viral Genomes
Viruses with RNA Genomes
Inhibitors of Virion Maturation and Assembly

