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Updated: Mar 27, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
[Vacuolating autotransporter toxin affects biological characteristics and pathogenicity of avian pathogenic
Objective:
To determined the role of vacuolating autotransporter toxin (vat) gene in avian pathogenic Escherichia coli (APEC), we detected the biological characteristics and pathogenicity of vat gene mutant strain of APEC-O1.
Methods:
We constructed the vat mutant and complementary strain of APEC-O1 by the Red recombination system and plasmid pSTV28. Then we compared the growth curve, motility, agglutination, biofilm formation and pathogenicity of mutant strain, wild-type strain and complementary strain.
Results:
The vat mutant did not affect the growth and resistance to environment stress of APEC. However, inactivation of APEC-O1 vat gene resulted in enhanced motility, diminished agglutination, decreased biofilm formation and attenuated virulence in ducks.
Conclusion:
These data indicated that Vat affect the motility, agglutination, biofilm formation and virulence of APEC-O1, which help us to understand the role of the Vat in the APEC pathogenicity.
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