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Updated: Mar 27, 2026
Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
Published on: May 1, 2018
[Efficacy of RNA interference mediated by cationic liposomes]
Abstract:
To investigate the cytotoxicity of the homemade peptide cationic liposome CDO14 and its efficacy of RNA interference (RNAi). MTT method was used to determine the cytotoxicity of the liposome to a human lung cancer cell line Luc-A549 that can express luciferase stably. Luciferase siRNA (Luc-siRNA) was transfected into Luc-A549 cells by CDO14. Contents of luciferase in the transfected cells were detected by luminous instrument and contents of total protein in these cells were detected by BCA method. Nude mice were inoculated with Luc-A549 cells in axilla to establish xenograft tumor model. Complexes of Luc-siRNA and the cationic liposomes were injected into the modeling mice via tail vein. Contents of luciferase in the transfected mice were detected by the whole body imaging system. The cytotoxicity of the homemade cationic liposome was similar to that of commercial liposome DOTAP, and lower than that of Lipo2000. The siRNA transfection efficacy mediated by CDO14 was higher than that mediated by DOTAP. The homemade peptide cationic liposome CDO14 is expected to serve as delivery vector in gene therapy because of its low cytotoxicity and high transfection efficiency.
Insights
A new peptide cationic liposome, CDO14, shows low cytotoxicity and high RNA interference efficiency in human lung cancer cells. This makes CDO14 a promising delivery vector for gene therapy applications.
Area of Science:
- Biotechnology
- Nanomedicine
- Gene Therapy
Background:
- RNA interference (RNAi) is a powerful tool for gene silencing.
- Effective delivery vectors are crucial for successful RNAi-based therapies.
- Homemade cationic liposomes offer potential advantages in drug delivery.
Purpose of the Study:
- To evaluate the cytotoxicity of a novel homemade peptide cationic liposome, CDO14.
- To assess the RNA interference (RNAi) efficacy of CDO14 as a delivery vector.
- To determine the potential of CDO14 in gene therapy applications.
Main Methods:
- Cytotoxicity was assessed using the MTT assay on human lung cancer cells (Luc-A549).
- RNA interference was achieved by transfecting Luc-siRNA into cells using CDO14.
- In vivo studies involved a xenograft tumor model in nude mice, with luciferase detection via whole-body imaging.
Main Results:
- CDO14 exhibited comparable cytotoxicity to commercial DOTAP and lower cytotoxicity than Lipo2000.
- CDO14 demonstrated superior siRNA transfection efficiency compared to DOTAP.
- In vivo imaging confirmed successful gene silencing in the tumor model.
Conclusions:
- The homemade peptide cationic liposome CDO14 possesses low cytotoxicity.
- CDO14 exhibits high transfection efficiency, outperforming DOTAP.
- CDO14 is a promising candidate for a delivery vector in gene therapy.
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