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Related Concept Videos

Transcription01:17

Transcription

35.4K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
35.4K
Transcription01:10

Transcription

158.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
158.8K
Transcription01:17

Transcription

3.4K
3.4K
Transcription01:10

Transcription

47.8K
47.8K
Transcription Initiation01:47

Transcription Initiation

22.0K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
22.0K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

25.2K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.2K

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Related Experiment Video

Updated: Mar 27, 2026

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
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Imaging Transcription: Past, Present, and Future.

Robert A Coleman1, Zhe Liu2, Xavier Darzacq3

  • 1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461.

Cold Spring Harbor Symposia on Quantitative Biology
|January 15, 2016
PubMed
Summary

Single-molecule live-cell imaging offers a dynamic view of gene transcription, overcoming limitations of traditional methods. This approach reveals real-time molecular events for predictive modeling of transcriptional regulation.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biophysics

Background:

  • Gene transcription is crucial for development and homeostasis, requiring precise regulation in eukaryotes.
  • Traditional methods offer static snapshots, failing to capture the dynamic nature of transcription.
  • Single-molecule live-cell imaging provides real-time, in-situ observation of transcriptional processes.

Purpose of the Study:

  • To review the evolution and current status of transcription imaging technologies.
  • To highlight key insights gained from single-molecule imaging of transcription.
  • To discuss future directions for resolving long-standing questions in transcriptional regulation.

Main Methods:

  • Utilizing advanced fluorescence microscopy and novel labeling techniques.
  • Observing molecular events in real-time within living cells and animals.
  • Quantifying transcriptional processes at single-molecule resolution.

Main Results:

  • Single-molecule imaging overcomes limitations of population-based studies.
  • Real-time observation enables derivation of cause-and-effect relationships.
  • Quantitative kinetic data facilitates the development of predictive models for transcription.

Conclusions:

  • Transcription imaging technologies have significantly advanced our understanding of gene regulation.
  • Live-cell, single-molecule approaches are essential for studying dynamic biological processes.
  • Future technological developments promise to further unravel complex transcriptional mechanisms.