Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Occupational airborne allergic contact dermatitis to invasive Compositae species treated with abrocitinib: A case report.

Contact dermatitis·2022

Same author

Excipient and Dose per Unit Area Affect Sensitivity When Patch Testing With Gold Sodium Thiosulfate.

Dermatitis : contact, atopic, occupational, drug·2018

Same journal

Reply to: Acrylate Copolymers/Crosspolymers in Sunscreens: Minimally Allergenic, Without Evidence of Need for Avoidance by (Meth)acrylate-Sensitized Individuals.

Contact dermatitis·2026

Same journal

Contact Allergy and Allergic Contact Dermatitis From Propylene Glycol and Related Glycols: Cosmetic Skin Sensitisers After All?

Contact dermatitis·2026

Same journal

Acrylate Copolymers/Crosspolymers in Sunscreens: Minimally Allergenic, Without Evidence of Need for Avoidance by (Meth)Acrylate-Sensitized Individuals.

Contact dermatitis·2026

Same journal

AI-Assisted Automated Two-Stage Patch Test Interpretation System Using Vision Transformer.

Contact dermatitis·2026

Same journal

Use of Tralokinumab in a Hairdresser With Severe Chronic Hand Eczema and Occupational Allergic Contact Dermatitis.

Contact dermatitis·2026

Same journal

Occupational Allergic Contact Dermatitis Caused by Cyclohexyl Diethanolamine in a Metalworking Fluid.

Contact dermatitis·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 27, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

PTBP-FR and TRUE Test®

Curt P Hamann¹

¹SmartPractice, Phoenix, AZ, 85008, USA.

Contact Dermatitis

|January 15, 2016

Summary

No abstract available in PubMed .

More Related Videos

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

Related Experiment Videos

Last Updated: Mar 27, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024