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Related Experiment Video

Updated: Mar 27, 2026

Extraction and Analysis of Cortisol from Human and Monkey Hair
08:38

Extraction and Analysis of Cortisol from Human and Monkey Hair

Published on: January 24, 2014

29.3K

A modified and cost-effective method for hair cortisol analysis.

Lianbin Xiang1, Imran Sunesara1, Kristina E Rehm1

  • 1a Laboratory of Behavioral Immunology Research , Division of Clinical Immunology and Allergy, Department of Medicine, Center of Biostatistics and Bioinformatics, University of Mississippi Medical Center , Jackson , MS , USA.

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
|January 16, 2016
PubMed
Summary

This study presents a new, affordable method for measuring hair cortisol, a key indicator of chronic psychological stress. The optimized technique uses simple equipment, making stress research more accessible.

Keywords:
Chronic stressevaporationextractionhair cortisolhair powder

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Last Updated: Mar 27, 2026

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Area of Science:

  • Biochemistry
  • Psychoneuroendocrinology
  • Analytical Chemistry

Background:

  • Hair cortisol analysis is a promising biomarker for assessing chronic psychological stress.
  • Existing methods for hair cortisol preparation can be costly and complex.
  • There is a need for accessible and cost-effective techniques for wider research application.

Purpose of the Study:

  • To develop and validate a modified, cost-effective method for preparing hair samples for cortisol assay.
  • To optimize conditions for cortisol extraction and evaporation from hair powder.

Main Methods:

  • Hair samples were ground using an inexpensive ball grinder (ULTRA-TURRAX tube drive).
  • Cortisol extraction was performed under various defined conditions.
  • Optimal extraction involved room temperature processing and evaporation using a stream of room air.

Main Results:

  • The modified method provides a cost-effective approach to hair sample preparation.
  • Optimal cortisol extraction was achieved at room temperature with air evaporation.
  • The technique is suitable for widespread research use.

Conclusions:

  • This economical method facilitates broader research into hair cortisol as a biomarker for chronic stress.
  • The validated technique can help researchers assess stress status more affordably.
  • Further validation of this method will support its clinical and research utility.