Amniotic fluid total antioxidant capacity and nitric oxide in emergency cerclage

Nitric oxide (NO) is one of the reactive oxygen species (ROS) that has been associated with inflammation. Total antioxidant capacity (TAC) neutralizes ROS. We evaluated that amniotic fluid (AF) TAC and NO correlate with the outcome of emergency cerclage.

Thirty-six women with cervical dilatation (≥2 cm) and bulging membranes between 16 and 24 weeks underwent emergency cerclage. Sixty-seven women between 16 and 24 weeks who had amniocentesis for chromosomal test provided control samples. AF samples were tested for TAC, and NO, and then correlated with pregnancy outcome.

AF TAC was significantly lower in cerclage group than control group (cerclage: 92.6 mmol/L versus control: 127.2 mmol/L, p < 0.001). Higher levels of AF TAC were associated with a longer latency from cerclage to delivery (r = 0.62, p < 0.001). NO was similar between two groups (p = 0.35). The mean gestational age at delivery of control group was better than cerclage group (cerclage: 29.5 weeks versus control: 39.4 weeks, p < 0.01).

Higher levels of AF TAC are correlated with longer prolongation days after cerclage. However, AF NO and iNOS are not different between two groups.