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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 27, 2026

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

Advancing Surgical Vision with Fluorescence Imaging.

Maximilian Koch^1,2, Vasilis Ntziachristos^1,2

¹Helmholtz Zentrum München, German Research Center for Environmental Health, Institute for Biological and Medical Imaging, 85764 Neuherberg, Germany;

Annual Review of Medicine

|January 16, 2016

Summary

This summary is machine-generated.

Intraoperative fluorescence imaging enhances surgical accuracy by revealing hidden disease and vital tissues. This technology offers real-time feedback, improving surgical precision and decision-making beyond human vision limitations.

Keywords:

fluorescence agents intraoperative imaging molecular imaging sentinel lymph node mapping targeted fluorescence imaging

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Area of Science:

Medical Imaging
Surgical Technology
Oncology

Background:

Human vision has limitations in detecting subsurface disease and differentiating between cancerous and healthy tissues due to low contrast.
Accurate intraoperative detection of disease and vital structures is crucial for surgical success.

Purpose of the Study:

To review and compare two main directions in intraoperative fluorescence imaging.
To highlight the potential of fluorescence imaging to improve surgical vision and decision-making.

Main Methods:

Comparison of fluorescence imaging using nonspecific dyes (for perfusion and viability) versus targeted agents (for disease interrogation).
Discussion of detection sensitivity challenges and differing strategies for each approach.

Main Results:

Fluorescence imaging provides surgeons with real-time feedback, enhancing visualization beyond human visual capabilities.
Two distinct approaches exist: nonspecific dyes for tissue assessment and targeted agents for disease-specific detection.

Conclusions:

Intraoperative fluorescence imaging offers a powerful tool to overcome the limitations of human vision in surgery.
This technology can seamlessly integrate into surgical workflows, improving precision and aiding interventional decisions.