Related Experiment Video
Updated: Mar 27, 2026
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Published on: May 17, 2024
Toll-like Receptor Expression Profile of Human Dental Pulp Stem/Progenitor Cells
Karim M Fawzy El-Sayed1, Pauline Klingebiel2, Christof E Dörfer2
1Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany; Oral Medicine and Periodontology Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt.
Introduction:
Human dental pulp stem/progenitor cells (DPSCs) show remarkable regenerative potential in vivo. During regeneration, DPSCs may interact with their inflammatory environment via toll-like receptors (TLRs). The present study aimed to depict for the first time the TLR expression profile of DPSCs.
Methods:
Cells were isolated from human dental pulp, STRO-1-immunomagnetically sorted, and seeded out to obtain single colony-forming units. DPSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, and CD146 expression and for their multilineage differentiation potential. After incubation of DPSCs in basic or inflammatory medium (interleukin-1β, interferon-γ, interferon-α, tumor necrosis factor-α), TLR expression profiles were generated (DPSCs and DPSCs-i).
Results:
DPSCs showed all characteristics of stem/progenitor cells. In basic medium DPSCs expressed TLRs 1-10 in different quantities. The inflammatory medium upregulated the expression of TLRs 2, 3, 4, 5, and 8, downregulated TLRs 1, 7, 9, and 10, and abolished TLR6.
Conclusions:
The current study describes for the first time the distinctive TLR expression profile of DPSCs in uninflamed and inflamed conditions.
More Related Videos
14:52Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
Published on: November 24, 2012
03:45Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
Published on: May 5, 2023