Flavobacterium lutivivi sp. nov., isolated from activated sludge

A Gram-stain-negative, strictly aerobic, yellow, rod-shaped bacterium, designated strain HQQ^T, was isolated from a municipal wastewater treatment plant in Hebei Province, PR China. Comparative 16S rRNA gene sequence analyses showed that strain HQQ^T is a member of the genus Flavobacterium and is closely related to 'Flavobacterium shanxiense' CCTCC AB 2014079^T (94.8 %) and Flavobacterium macrobrachii DSM 22219^T (94.7 %). Phylogenetic analysis showed that strain HQQ^T clustered with Flavobacterium fontis JCM 18212^T and Flavobacterium squillarum KCTC 23915^T. The polar lipid profile of strain HQQ^T revealed the presence of phosphatidylethanolamine, six unknown aminolipids, one unknown glycolipid and one unknown lipid and the only isoprenoid quinone was MK-6. The dominant fatty acids of strain HQQ^T were iso-C_15 : 0, C_15 : 0 and C_16 : 1ω7c. The DNA G+C content of strain HQQ^T is 32 mol%. On the basis of the phylogenetic and phenotypic data, strain HQQ^T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium lutivivi sp. nov. is proposed. The type strain is HQQ^T ( = CGMCC 1.15347^T = KCTC 42935^T).