Paenibacillus bovis sp. nov., isolated from raw yak (Bos grunniens) milk

Strain BD3526^T, isolated from raw yak milk collected in Tibet, China, was studied to determine its taxonomic status. The strain was Gram-reaction positive, motile, catalase-positive, aerobic or facultatively anaerobic. The DNA G+C content of the strain was 47.5 mol% and its peptidoglycan contained meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C_15 : 0, anteiso-C_17 : 0 and C_16 : 0. Based on 16S rRNA gene sequence analysis, similarities among strain BD3526^T and its most closely related species, Paenibacillus shenyangensis A9^T, 'Paenibacillus dauci' H9, 'Paenibacillus wulumuqiensis' Y24 and Paenibacillus hunanensis DSM 22170^T were 99.0, 98.5, 97.3 and 96.7 %, respectively. Levels of DNA-DNA relatedness among strain BD3526^T and P. hunanensis DSM 22170^T and Paenibacillus polymyxa ATCC 842^T ( = DSM 36^T), the type species of the genus, were 41.2 and 45.6 %, respectively. In silico genome-to-genome comparison showed that the DNA-DNA hybridization values among strain BD3526^T and P. shenyangensis A9^T, 'P. dauci' H9 and 'P. wulumuqiensis' Y24 were lower than 70 %. Based on its molecular and physiological properties, strain BD3526^T ( = DSM 28815^T = CGMCC 8333^T) is identified as the type strain of a novel species within the genus Paenibacillus, for which the name Paenibacillus bovis is proposed.