Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

17
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
17
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.2K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pseudogenization of the cntQ permease confers distinct yersinopine-metal uptake selectivity in Yersinia species.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine·2026
Same author

Emerging neurological and cognitive symptoms in patients with late-onset ornithine transcarbamylase deficiency: a narrative review.

Metabolic brain disease·2026
Same author

Exploring Sex and Clinical Factors Associated with Long-Term Survival After Implantable Cardioverter-Defibrillator Implantation: A 10-Year Cohort Study.

Journal of clinical medicine·2026
Same author

Quantitative mapping of methionine sensitivity to oxidation in the copper-bound PcuC chaperone.

Redox biology·2026
Same author

Readability Analysis of Commonly Cited Patient-Reported Outcome Measures in the Orthopaedic Joint Literature.

Arthroplasty today·2026
Same author

Transforming One Health in India: National Multisectoral Mixed Method Study on Prioritization of Zoonotic Diseases.

JMIR public health and surveillance·2025

Related Experiment Video

Updated: Mar 27, 2026

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples
09:51

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

579

A bioluminescent arsenite biosensor designed for inline water analyzer.

Sandra Prévéral1,2,3, Catherine Brutesco1,2,3, Elodie C T Descamps1,2,3

  • 1CEA, DSV, IBEB, Lab Bioenerget Cellulaire, Saint-Paul-lez-Durance, F-13108, France.

Environmental Science and Pollution Research International
|January 16, 2016
PubMed
Summary
This summary is machine-generated.

Lyophilization preserves whole-cell biosensors for water quality monitoring. Lyophilized Escherichia coli biosensors maintain sensitivity to arsenite and general toxicity for field use.

Keywords:
BioluminescenceBiosensorsLyophilization

More Related Videos

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.9K
Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors
07:13

Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors

Published on: November 15, 2016

10.7K

Related Experiment Videos

Last Updated: Mar 27, 2026

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples
09:51

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

579
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.9K
Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors
07:13

Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors

Published on: November 15, 2016

10.7K

Area of Science:

  • Environmental microbiology
  • Biosensor technology
  • Reporter gene systems

Background:

  • Whole-cell biosensors offer rapid detection of water contaminants.
  • Current limitations include bacterial conservation for autonomous field devices.
  • Lyophilization is explored to improve biosensor stability.

Purpose of the Study:

  • To assess the impact of lyophilization on Escherichia coli-based whole-cell biosensors.
  • To evaluate the shelf life and performance of lyophilized biosensors for water analysis.

Main Methods:

  • Two reporter gene biosensors (general toxicity and arsenite detection) in E. coli were lyophilized.
  • Lyophilized cells were stored at 4°C for up to a year.
  • Revival rates and biosensor performance (sensitivity, specificity, response time) were evaluated.

Main Results:

  • Approximately 40% of lyophilized cells could be revived after storage.
  • Lyophilized arsenite biosensors maintained performance after 7 months, with a 0.2 μM detection limit.
  • Response times were approximately one hour with good reproducibility.

Conclusions:

  • Lyophilization is a viable method for preserving whole-cell biosensors for water monitoring.
  • Lyophilized biosensors can be used as stable consumables in autonomous field analyzers.
  • This approach supports the development of robust systems for real-time water quality surveillance.