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Updated: Mar 27, 2026
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Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
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Novel inexpensive fungi proteases: Production by solid state fermentation and characterization
Paula Kern Novelli1, Margarida Maria Barros2, Luciana Francisco Fleuri3
1FMVZ, UNESP (University of São Paulo State), Department of Chemistry and Biochemistry, IB, Botucatu, SP, Brazil.
Food Chemistry
|January 16, 2016
Summary
Agroindustrial residues enable fungal protease production with diverse biochemical traits. Aspergillus oryzae proteases exhibit high stability and activity, offering biotechnological potential.
Area of Science:
- Biotechnology
- Enzymology
- Microbiology
Background:
- Solid-state fermentation (SSF) offers a sustainable method for enzyme production.
- Agroindustrial residues are abundant, low-cost substrates for microbial processes.
- Fungal proteases are valuable industrial enzymes with diverse applications.
Purpose of the Study:
- To compare protease production by various fungal strains using agroindustrial residues.
- To characterize the biochemical properties of the produced proteases.
- To evaluate the potential of fungal proteases for biotechnological applications.
Main Methods:
- Solid-state fermentation (SSF) using agroindustrial residues as substrate.
- Cultivation of multiple fungal strains, including Aspergillus oryzae, Penicillium roquefortii, and Aspergillus flavipes.
- Characterization of protease activity, optimal pH, temperature stability, and yield.
Main Results:
- High protease production was achieved from most fungal strains studied.
- Produced proteases exhibited significant variations in optimal pH and temperature stability.
- Aspergillus oryzae demonstrated stability across all tested pH values.
- Penicillium roquefortii and Aspergillus flavipes showed optimal activity at 50 °C and 90 °C, respectively.
- Lyophilized protease from A. oryzae yielded 1251.60 U/g and 155010.66 U/kg of substrate.
Conclusions:
- Substrate and fungal strain significantly influence the biochemical characteristics of proteases.
- Fungal proteases produced via SSF exhibit high activity and stability.
- These proteases represent cost-effective alternatives for the biotechnological industry.
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