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Changes in platelet function following cold storage of RBC suspensions
Qian-Li Dang1, Jian-Gang Li2, Yang Sun1
1Department of Transfusion Medicine, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China.
International Journal of Clinical and Experimental Medicine
|January 16, 2016
Summary
Human platelets stored in red blood cell suspensions at 4°C for 21 days maintain high function. This finding supports cold storage protocols for platelet research and clinical applications.
Area of Science:
- Hematology
- Transfusion Medicine
- Platelet Biology
Background:
- Platelet storage for transfusion and research is critical.
- Current cold storage methods can impact platelet function.
- Standardizing platelet storage is essential for reliable experimental outcomes.
Purpose of the Study:
- To establish a basis for cold-storing human platelets.
- To evaluate changes in platelet function after cold storage.
- To determine optimal conditions for preserving platelet activity.
Main Methods:
- Human platelet function was assessed in red blood cell suspensions (RBCs).
- Platelets were stored at 4°C for up to 28 days.
- Platelet activation (CD62P) and thromboelastography (TEG) were measured.
Main Results:
- After 21 days of cold storage, platelets in RBC suspensions retained significant function.
- Activated platelets (PAC1+62P+, PAC1-62P+) showed normal TEG-maximum amplitude (TEG-MA) indices.
- A substantial proportion of platelets maintained their functional capacity.
Conclusions:
- Platelets in RBC suspensions exhibit high activity after 21 days of cold storage at 4°C.
- This study provides crucial data for protocols involving cold storage of platelets.
- Findings support the potential for extended cold storage of functional human platelets.
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