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miR-503 inhibits cell proliferation and invasion in glioma by targeting L1CAM
Hao Liu1, Zhi Song2, Daguang Liao1
1Department of Neurosurgery, The Third Xiangya Hospital of Central South University Changsha 410013, Hunan, P. R. China.
Abstract:
Deregulated microRNAs and their roles in tumorigenesis have attracted much attention in recent years. Although miR-503 has been reported to be aberrant expression in several cancers, its role in glioma remains unknown. In this study, we focused on the expression and mechanisms of miR-503 in glioma development. We found that miR-503 was downregulated in glioma cell lines and tumor tissues, and the restoration of miR-503 reduced cell proliferation invasion. Furthermore, bioinformatics analysis indicated that L1CAM was a putative target of miR-503. In a Luciferase reporter system, we confirmed that L1CAM was a direct target gene of miR-503. These findings indicate that miR-503 suppresses glioma cell growth by negatively regulating the expression of L1CAM. Collectively, our data identify the important roles of miR-503 in glioma pathogenesis, indicating its potential application in cancer therapy.
Insights
MicroRNA-503 (miR-503) is downregulated in glioma, suppressing tumor growth and invasion by targeting L1CAM. Restoring miR-503 shows potential for glioma cancer therapy.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) play crucial roles in tumorigenesis.
- miR-503 has shown aberrant expression in various cancers, but its function in glioma is unclear.
Purpose of the Study:
- To investigate the expression and functional role of miR-503 in glioma development.
- To identify the molecular mechanisms underlying miR-503's action in glioma.
Main Methods:
- Quantitative real-time PCR to assess miR-503 expression in glioma cell lines and tissues.
- Cell proliferation and invasion assays.
- Bioinformatics analysis to predict miR-503 targets.
- Luciferase reporter assays to validate L1CAM as a direct target of miR-503.
Main Results:
- miR-503 expression was significantly downregulated in glioma cell lines and tumor tissues.
- Restoration of miR-503 inhibited glioma cell proliferation and invasion.
- L1CAM was identified as a direct target gene of miR-503.
- miR-503 negatively regulates L1CAM expression.
Conclusions:
- miR-503 acts as a tumor suppressor in glioma by targeting L1CAM.
- These findings highlight the critical role of miR-503 in glioma pathogenesis.
- miR-503 represents a potential therapeutic target for glioma treatment.
