Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

38.9K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.9K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.9K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.9K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

3.5K
3.5K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.4K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.4K
The Cell Cycle Control System01:28

The Cell Cycle Control System

6.4K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting of Gpx8-CSF1 axis resets the immune milieu of lung tumor and overcomes resistance to anti-PD-1 therapy.

Cell death and differentiation·2026
Same author

Mapping the human female reproductive tract.

Nature cell biology·2026
Same author

Deciphering O‑GlcNAc-Dependent Signaling Via Integrated Proteomics and Phosphoproteomics.

ACS omega·2026
Same author

Inpatient Management of Benzodiazepine Withdrawal: A Narrative Review of a Systematic, Individualized Taper Design.

Harvard review of psychiatry·2026
Same author

PD-1 regulates latent effector differentiation of thymic cytotoxic CD8<sup>+</sup> T cells.

Nature communications·2026
Same author

Correction to: Autocrine activation of JAK2 by IL-11 promotes platinum drug resistance.

Oncogene·2026

Related Experiment Video

Updated: Mar 27, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

28.7K

MMSET is dynamically regulated during cell-cycle progression and promotes normal DNA replication.

Debra L Evans1, Haoxing Zhang2,3, Hyoungjun Ham4

  • 1a Mayo Graduate School, Biochemistry and Molecular Biology (BMB) Track, Mayo Clinic , Rochester , MN , USA.

Cell Cycle (Georgetown, Tex.)
|January 16, 2016
PubMed
Summary

Multiple myeloma SET domain-containing protein (MMSET) is degraded during S phase, impacting DNA replication. This study reveals MMSET

Keywords:
Cdt2DNA replicationE3 ubiquitin ligaseMMSETcell cycleproliferating cell nuclear antigen (PCNA)ubiquitin

More Related Videos

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

15.1K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.7K

Related Experiment Videos

Last Updated: Mar 27, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

28.7K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

15.1K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.7K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Biology

Background:

  • Cellular DNA replication is tightly regulated for genome integrity.
  • The Origin Recognition Complex (ORC) and pre-replication complex (pre-RC) coordinate DNA replication initiation.
  • Multiple myeloma SET domain-containing protein (MMSET) is a histone methyltransferase implicated in cancer and development.

Purpose of the Study:

  • To investigate the cell-cycle regulation of MMSET.
  • To determine the role of MMSET in DNA replication and cell-cycle progression.

Main Methods:

  • Investigated MMSET degradation during the cell cycle.
  • Utilized cullin-ring ligase 4-Cdt2 (CRL4(Cdt2)) and proteasome-dependent assays.
  • Assessed DNA replication and pre-RC factor association with chromatin in MMSET-depleted cells.

Main Results:

  • MMSET is degraded during S phase via a CRL4(Cdt2) and proteasome-dependent pathway.
  • MMSET depletion causes defects in DNA replication.
  • Reduced association of pre-RC factors with chromatin was observed in MMSET-depleted cells.

Conclusions:

  • MMSET levels are dynamically regulated throughout the cell cycle.
  • MMSET plays a crucial role in DNA replication and cell-cycle progression.
  • Findings provide new insights into MMSET function in cancer and development.