Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

9.8K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
9.8K
Types Of Column Chromatography01:29

Types Of Column Chromatography

15.1K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
15.1K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

8.2K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities.

The Plant cell·2026
Same author

Hetero-oligomerization drives structural plasticity of eukaryotic peroxiredoxins.

Nature chemical biology·2026
Same author

Integrating natural variation through GWAS - genetics of drought and flood tolerance in grass pea reveal independent yet interconnected mechanisms.

BMC plant biology·2026
Same author

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity.

Molecular oncology·2026
Same author

Pervanadate-induced oxidation relieves autoinhibition of the protein tyrosine kinase SRC.

Science signaling·2026
Same author

Single-cell imaging of liver metabolic dynamics using fluorescent biosensors.

Trends in endocrinology and metabolism: TEM·2025

Related Experiment Video

Updated: Mar 27, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.7K

Diagonal chromatography to study plant protein modifications.

Alan Walton1, Liana Tsiatsiani2, Silke Jacques1

  • 1Department of Medical Protein Research, VIB, 9000 Ghent, Belgium; Department of Biochemistry, Ghent University, 9000 Ghent, Belgium; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

Biochimica Et Biophysica Acta
|January 17, 2016
PubMed
Summary
This summary is machine-generated.

Diagonal chromatography offers versatile proteome research applications, enabling the study of plant protein modifications like protease processing, ubiquitination, and methionine oxidation through adaptable peptide separation techniques.

Keywords:
COFRADICChaFRADICDiagonal chromatographyMethionine oxidationMethionine sulfoxideProteaseProtein processingProtein ubiquitination

More Related Videos

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
13:02

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Published on: March 18, 2011

38.2K
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

12.1K

Related Experiment Videos

Last Updated: Mar 27, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.7K
Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
13:02

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Published on: March 18, 2011

38.2K
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

12.1K

Area of Science:

  • Proteomics
  • Biochemistry
  • Plant Science

Background:

  • Diagonal chromatography is a versatile technique for proteome research.
  • It allows for adaptable peptide modification or sorting steps between separations.
  • This adaptability facilitates the enrichment of specific peptide types.

Purpose of the Study:

  • To highlight the utility of diagonal chromatography in plant proteomics.
  • To demonstrate its application in studying various plant protein modifications.
  • To detail the specific sorting steps and results for different modification analyses.

Main Methods:

  • Utilizing diagonal chromatography for sequential peptide separations.
  • Implementing tailored peptide sorting/modification steps for specific analyses.
  • Focusing on applications in plant protein research.

Main Results:

  • Demonstrated successful application of diagonal chromatography for studying protease-processed proteins.
  • Showcased its effectiveness in analyzing protein ubiquitination in plants.
  • Presented results on the oxidation of protein-bound methionines using this technique.

Conclusions:

  • Diagonal chromatography is a powerful and flexible tool for plant proteomics.
  • It enables in-depth investigation of diverse protein modifications.
  • The method facilitates the enrichment and analysis of specific peptide subsets.