Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.9K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.9K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

26.0K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
26.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Labeling DNA Probes03:31

Labeling DNA Probes

9.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct visualization of native GSDMD pores reveals lipid-driven stabilization during pyroptosis.

Science advances·2026
Same author

Membrane protein solubilization and structure determination using de novo-designed proteins.

Science (New York, N.Y.)·2026
Same author

ILK binding to β1 integrin is indirect and mediated by kindlin-2.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Nuclear proteome reveals microtubule-associated protein regulating fate and disease.

Cell·2026
Same author

De novo design of miniproteins targeting GPCRs.

Nature·2026
Same author

The past, present and future of de novo protein design.

Nature·2026

Related Experiment Video

Updated: Mar 27, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K

DNA nanotechnology and fluorescence applications.

Thomas Schlichthaerle1, Maximilian T Strauss1, Florian Schueder1

  • 1Department of Physics and Center for Nanoscience, Ludwig Maximilian University, 80539 Munich, Germany; Max Planck Institute of Biochemistry, 82152 Martinsried near Munich, Germany.

Current Opinion in Biotechnology
|January 17, 2016
PubMed
Summary

Structural DNA nanotechnology uses DNA

More Related Videos

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

23.1K
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

15.7K

Related Experiment Videos

Last Updated: Mar 27, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K
Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

23.1K
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

15.7K

Area of Science:

  • DNA nanotechnology
  • Nanoscale science
  • Molecular engineering

Background:

  • DNA's unique molecular recognition properties enable precise nanoscale construction.
  • DNA nanostructures act as molecular breadboards for organizing guest molecules.
  • These assemblies offer control over molecular identity, number, and spacing.

Purpose of the Study:

  • To introduce structural DNA nanotechnology.
  • To discuss applications in fluorescence and plasmonics.
  • To highlight the potential for studying biological phenomena with nanoscale precision.

Main Methods:

  • Utilizing DNA's self-assembly properties.
  • Designing complex 2D and 3D nanostructures.
  • Integrating guest molecules like proteins, fluorophores, and nanoparticles.

Main Results:

  • Demonstrated construction of complex DNA nanostructures.
  • Enabled precise placement of guest molecules.
  • Facilitated controlled studies of biological phenomena.

Conclusions:

  • Structural DNA nanotechnology provides a powerful platform for nanoscale engineering.
  • DNA nanostructures are versatile tools for applications in fluorescence and plasmonics.
  • This field offers unprecedented control for investigating biological systems.