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Microfluidic immunomagnetic cell separation from whole blood.

Sajay Bhuvanendran Nair Gourikutty1, Chia-Pin Chang2, Poenar Daniel Puiu3

  • 1BioElectronics Programme, Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 1 Science Park Road, 117528, Singapore; NOVITAS-Centre for Micro-/Nano-electronics, School of Electrical and Electronics Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|January 17, 2016
PubMed
Summary

This study presents an efficient microfluidic device for high-throughput cell separation using immunomagnetic techniques. The device achieves over 99.9% capture efficiency for white blood cells (WBCs) from whole blood.

Keywords:
Cell separationMagnetic forceMagnetic particlesMagnetic separationMagnetophoresisNegative enrichmentTrapping

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Separation Technologies

Background:

  • Immunomagnetic-based separation is a key technique for isolating cells and biomolecules.
  • Microfluidic devices offer advantages in throughput and efficiency for cell manipulation.

Purpose of the Study:

  • To design and analyze a microfluidic device for high-throughput, high-efficiency capture of magnetically tagged cells.
  • To demonstrate proof-of-concept by separating white blood cells (WBCs) from whole human blood.

Main Methods:

  • Developed a microfluidic chip with customized permanent magnet arrays to create large magnetic field gradients.
  • Utilized a finite element analysis (FEA) model to simulate and validate the cell separation process.
  • Employed CD45-conjugated magnetic particles to label WBCs in whole blood samples for separation.

Main Results:

  • Achieved effective capture of magnetically tagged cells by manipulating magnetic and fluid dynamic forces under laminar flow.
  • FEA simulations accurately predicted device behavior, validated by experimental results.
  • Demonstrated over 99.9% capture efficiency for WBCs from whole human blood.

Conclusions:

  • The proposed microfluidic device provides a simple and efficient method for high-throughput cell separation.
  • The design is versatile and can be applied to both positive selection and negative enrichment of rare cells.
  • This technology holds promise for various applications in cell biology and clinical diagnostics.