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Published on: June 29, 2017
A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides,
Jian-Long Xue1, Shuai Zhao1, Rui-Ming Liang1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China.
Abstract:
In this study, a co-production of two high value-added products, glucose and xylooligosaccharides (XOS), was investigated by utilizing sugarcane bagasse (SB) within a multi-product bio-refinery framework optimized by Box-Behnken design-based response surface methodology. The developed process resulted in a maximum cellulose conversion of xylan-removed SB, 98.69±1.30%, and a maximum extracted SB xylan conversion into XOS (xylobiose and xylotriose) of 57.36±0.79% that was the highest SB xylan conversion reported in the literature, employing cellulase from Penicillium oxalicum EU2106 and recombinant endo-β-1,4-xylanase in Pichia pastoris. Consequently, a mass balance analysis showed that the maximum yields of glucose and XOS were 34.43±0.32g and 5.96±0.09 g per 100 g raw SB. Overall, this described process may be a preferred option for the comprehensive utilization of SB.
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