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Updated: Mar 27, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides
Daniela Verga1, Moritz Welter1, Andreas Marx1
1Department of Chemistry and Konstanz Research School Chemical Biology University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
Abstract:
DNA polymerases can efficiently and sequence selectively incorporate oligonucleotide (ODN)-modified nucleotides and the incorporated oligonucleotide strand can be employed as primer in rolling circle amplification (RCA). The effective amplification of the DNA primer by Φ29 DNA polymerase allows the sequence-selective hybridisation of the amplified strand with a G-quadruplex DNA sequence that has horse radish peroxidase-like activity. Based on these findings we develop a system that allows DNA detection with single-base resolution by naked eye.
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