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Updated: Mar 27, 2026
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Published on: December 8, 2023
Molecular characterization of novel immunodominant molybdenum cofactor biosynthesis protein C1 (Rv3111) from
Shubhra Srivastava1, Manisha Pathak2, Himanshu Pandey1
1Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India.
Background:
In the molybdenum cofactor biosynthesis pathway, MoaA and MoaC catalyze the first step of transformation of GTP to cPMP. In M. tuberculosis H37Rv, three different genes (Rv3111, Rv0864 and Rv3324c) encode for MoaC homologs. Out of these three only MoaC1 (Rv3111) is secretory in nature.
Methods:
We have characterized MoaC1 protein through biophysical, in-silico, and immunological techniques.
Results:
We have characterized the conformation and thermodynamic stability of MoaC1, and have established its secretory nature by demonstrating the presence of anti-MoaC1 antibodies in human tuberculosis patients' sera. Further, MoaC1 elicited a dominant Th1 immune response in mice characterized by increased induction of IL-2 and IFN-γ.
Conclusion:
Integrating these results, we conclude that MoaC1 is a structured secretory protein capable of binding with GTP and eliciting induced immune response.
General Significance:
This study would be useful for the development of vaccines against tuberculosis and to improve methods used for diagnosis of tuberculosis.
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