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Related Experiment Video

Updated: Mar 27, 2026

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts.

Oleg V Podgorny¹, Vassili N Lazarev²

¹Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia; Koltzov Institute of Developmental Biology of the Russian Academy of Sciences, 26 Vavilov Str., Moscow 119334, Russia.

Journal of Microbiological Methods

|January 18, 2016

Summary

Laser microdissection isolates specific cells for molecular analysis. This technique, though uncommon in microbiology, offers powerful insights into host-microorganism interactions and bacterial studies.

Keywords:

Biofilm Host–microorganism interactions Laser microdissection Single cell analysis

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Last Updated: Mar 27, 2026

Laser Microdissection for Species-Agnostic Single-Tissue Applications

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Published on: March 31, 2022

Laser Capture Microdissection of Mammalian Tissue

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Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

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Published on: May 10, 2016

Area of Science:

Microbiology
Molecular Biology
Cell Biology

Background:

Laser microdissection (LMD) enables isolation of pure cell populations from complex tissues.
LMD is established in various biological fields but underutilized in microbiology.
Previous studies show LMD's utility in host-pathogen interactions and bacterial research.

Purpose of the Study:

To detail the methodological aspects of laser microdissection instruments.
To showcase LMD's advantages for microbiological research through representative examples.
To encourage wider adoption of LMD in microbiology.

Main Methods:

Description of commercially available laser microdissection systems.
Application of LMD for isolating specific microbial or host cells from their native environment.
Downstream molecular analyses (genomics, transcriptomics, proteomics, metabolomics) of isolated cells.

Main Results:

LMD effectively isolates homogenous cell populations from diverse biological samples.
Demonstrated success in studying host-microorganism interactions in both animal and plant systems.
Facilitated investigation of bacteria in biofilms, identification of unculturable bacteria, and single-cell analysis.

Conclusions:

Laser microdissection is a valuable, versatile tool for microbiological research.
LMD overcomes limitations of traditional methods for studying microbial communities and interactions.
The paper provides a methodological overview and practical examples to promote LMD adoption in microbiology.