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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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The design and analysis of transposon insertion sequencing experiments

Michael C Chao1, Sören Abel2, Brigid M Davis1

  • 1Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts 02115, USA; the Division of Infectious Disease, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA; and the Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA.

Nature Reviews. Microbiology
|January 19, 2016
PubMed
Summary

Transposon insertion sequencing (TIS) identifies genes essential for bacterial growth. Careful experimental design and statistical analysis are crucial for accurate TIS data interpretation.

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