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Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number
J B March1, M D Colloms, D Hart-Davis
1Department of Molecular Biology, University of Edinburgh.
Molecular Microbiology
|July 1, 1989
Abstract:
A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.