Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

28.5K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
28.5K
Telomeres and Telomerase02:41

Telomeres and Telomerase

8.0K
8.0K
Replicative Cell Senescence02:15

Replicative Cell Senescence

4.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.6K
Replication in Eukaryotes01:29

Replication in Eukaryotes

18.9K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
18.9K
Replication in Eukaryotes02:31

Replication in Eukaryotes

207.3K
Overview
207.3K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.7K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual actionability of BMI1 activation and mitotic vulnerability defines adaptive Osimertinib resistance in EGFR-mutant NSCLC.

Cell death & disease·2026
Same author

Mutation type-specific transcriptomic signatures and readthrough therapy rescue in SMC1A-related developmental and epileptic encephalopathy.

Epilepsia·2026
Same author

Correction: An evolutionarily conserved role for separase in the regulation of nuclear lamins.

Cell death discovery·2026
Same author

Exosome-mediated decay of unstable long extended precursors of human telomerase RNA is dependent on 5'-cap trimethylation.

Genes & development·2026
Same author

Modulating TERRA G-quadruplexes with ligands: impact on telomeric DNA:RNA hybrids and ALT mechanisms.

Nucleic acids research·2025
Same author

Neddylation inhibition induces DNA double-strand breaks, hampering tumor growth in vivo, and promotes radiosensitivity in PAX3-FOXO1 rhabdomyosarcoma.

Cell death discovery·2025

Related Experiment Video

Updated: Mar 27, 2026

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions
11:21

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

1.4K

A role for Separase in telomere protection.

Francesca Cipressa1,2, Patrizia Morciano1,2, Giuseppe Bosso1,2

  • 1Department of Biology and Biotechnology "Charles Darwin" Section of Genetics, SAPIENZA University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.

Nature Communications
|January 19, 2016
PubMed
Summary

Drosophila Separase (Sse) mutations cause telomere fusions by reducing HP1 levels, a conserved mechanism also seen in human cells. This highlights Separase

More Related Videos

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
08:34

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Published on: April 13, 2015

10.8K
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

47.5K

Related Experiment Videos

Last Updated: Mar 27, 2026

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions
11:21

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

1.4K
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
08:34

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Published on: April 13, 2015

10.8K
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

47.5K

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Drosophila telomeres are protected by the terminin complex and other proteins.
  • Telomere maintenance in Drosophila differs from telomerase-driven mechanisms in many eukaryotes.

Purpose of the Study:

  • To investigate the role of Drosophila Separase (Sse) in telomere capping and protection.
  • To elucidate the molecular mechanisms underlying Sse-mediated telomere stability.

Main Methods:

  • Analysis of Sse mutants in Drosophila for endoreduplication and telomeric fusions.
  • Co-immunoprecipitation to assess binding of Separase to terminin proteins and HP1.
  • HP1 overexpression experiments in Sse mutants.
  • siRNA-mediated depletion of ESPL1 (human Separase orthologue) in primary fibroblasts.

Main Results:

  • Sse mutations lead to telomeric fusions (TFs), indicating a role in telomere capping.
  • Separase binds terminin proteins and HP1, and localizes to telomeres.
  • Loss of Sse significantly reduces HP1 levels, and HP1 overexpression suppresses TFs in Sse mutants.
  • Depletion of human ESPL1 causes telomere dysfunction and reduced HP1 levels.

Conclusions:

  • Drosophila Separase plays a crucial role in telomere protection, likely through maintaining HP1 levels at telomeres.
  • Telomeric fusions in Sse mutants are caused by HP1 diminution.
  • The role of Separase in telomere protection is conserved between Drosophila and humans.