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Related Experiment Video

Updated: Mar 27, 2026

Double Emulsion Generation Using a Polydimethylsiloxane PDMS Co-axial Flow Focus Device
08:58

Double Emulsion Generation Using a Polydimethylsiloxane PDMS Co-axial Flow Focus Device

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Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device.

Russell H Cole1, Tuan M Tran2, Adam R Abate3

  • 1Department of Pharmaceutical Chemistry, University of California, San Francisco.

Journal of Visualized Experiments : Jove
|January 19, 2016
PubMed
Summary

This study presents a polydimethylsiloxane (PDMS) microfluidic device for creating water/oil/water double emulsions. The novel device uses soft lithography and a 3D flow focusing geometry for precise control over emulsion size.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Chemical Engineering

Background:

  • Double emulsions are crucial for biological and industrial applications requiring aqueous carrier fluids.
  • Existing methods for generating double emulsions often face limitations in control and scalability.

Purpose of the Study:

  • To develop a polydimethylsiloxane (PDMS) microfluidic device for efficient water/oil/water double emulsion generation.
  • To demonstrate the fabrication of the device using soft lithography and a novel 3D flow focusing geometry.

Main Methods:

  • Fabrication of the microfluidic device using two-layer soft lithography to create a 3D flow focusing geometry.
  • Assembly of PDMS slabs with complementary locking features for accurate alignment.
  • Testing the device for double emulsion formation using coaxial flow focusing.

Main Results:

  • Successful generation of water/oil/water double emulsions with diameters ranging from 14 µm to 50 µm.
  • Demonstration of device fabrication entirely through soft lithography.
  • Formation of stable emulsions in large, clog-resistant channels.

Conclusions:

  • The developed PDMS microfluidic device offers a robust and scalable platform for producing double emulsions.
  • The 3D flow focusing geometry and soft lithography fabrication enable precise control over emulsion characteristics.
  • The device's design addresses limitations of previous methods, enhancing applicability in various fields.