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Nonclassical human leukocyte antigen (HLA-G, HLA-E, and HLA-F) in coronary artery disease
Ines Zidi1, Najla Kharrat2, Rania Abdelhedi2
1Laboratory Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
Insights
The HLA-G +3142C/G polymorphism and specific haplotypes are linked to an increased risk of coronary artery disease (CAD) in the Tunisian population, suggesting a role for these genes in CAD development.
Area of Science:
- Immunogenetics
- Cardiovascular Disease Research
Background:
- Coronary artery disease (CAD) is inflammatory and linked to thrombosis.
- Human Leukocyte Antigen (HLA) genes play a role in immune responses and disease susceptibility.
Purpose of the Study:
- To investigate the association between HLA-G, HLA-E, and HLA-F genetic polymorphisms and CAD in a Tunisian cohort.
- To evaluate the diagnostic value of these polymorphisms for CAD risk.
Main Methods:
- Genotyping of specific HLA-G, HLA-E, and HLA-F polymorphisms in 89 CAD patients and 84 controls.
- Analysis of single nucleotide polymorphisms and haplotype associations.
Main Results:
- A significant association was found between the HLA-G +3142 G allele and an increased risk of CAD (OR=1.64, p=0.02).
- TDELCA and TDELGG haplotypes were also significantly associated with CAD (p=0.008 and p=0.030, respectively).
- No significant associations were observed for other studied polymorphisms.
Conclusions:
- The HLA-G +3142C/G polymorphism and TDELCA/TDELGG haplotypes may serve as reliable diagnostic markers for CAD risk.
- HLA-G, -E, and -F molecules are potentially involved in the pathogenesis of coronary artery disease.
- Further research is needed to validate these findings.
Aims:
Several evidences suggest the association between the evolution of coronary artery disease (CAD) and the development of coronary syndrome that is often associated with disrupted plaque and partial or complete thrombosis of the related artery. Because of the inflammatory nature of CAD, we investigated the human leukocyte antigen (HLA)-G, HLA-E, and HLA-F genetic polymorphisms within CAD patients and evaluated their potential association with this disease in Tunisian population.
Methods:
Different polymorphisms in HLA-G (14-bp Insertion/Deletion, +3142C/G), HLA-E (HLA-E*01:01/01:03 A/G), HLA-F (HLA-F*01:02 T/C, 01:03 C/T, 01:04 A/C) genes were typed using different laboratory techniques in a cohort of 89 CAD patients and 84 controls.
Results:
A significant association was reported between the HLA-G +3142 G allele (OR=1.64, 95% CI=1.05-2.56, p=0.02) and increased risk of CAD. No association was found for the other studied polymorphisms. When we considered the haplotypes, we found TDELCA and TDELGG haplotypes associated to CAD with p=0.008 and p=0.030, respectively, suggesting the potential interaction between HLA-G and HLA-E genes.
Conclusions:
Our findings indicated that the HLA-G +3142C/G polymorphism and TDELCA and TDELGG haplotypes can harbour a reliable diagnosis value for the risk of CAD development suggesting that HLA-G, -E and -F molecules might be involved in the pathogenesis of the disease. However, further studies are necessary to confirm our results.
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