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Updated: Jul 28, 2026

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Published on: March 25, 2009
A simple protocol for attenuating the auto-fluorescence of cyanobacteria for optimized fluorescence in situ
Perrine Zeller1, Olivier Ploux2, Annick Méjean3
1INSERM U1193, Physiopathogénèse et Traitement des Maladies du Foie, Hôpital Paul Brousse, bâtiment Lavoisier, 14 avenue Paul Vaillant Couturier, 94807 Villejuif Cedex, France.
Abstract:
Cyanobacteria contain pigments, which generate auto-fluorescence that interferes with fluorescence in situ hybridization (FISH) imaging of cyanobacteria. We describe simple chemical treatments using CuSO4 or H2O2 that significantly reduce the auto-fluorescence of Microcystis strains. These protocols were successfully applied in FISH experiments using 16S rRNA specific probes and filamentous cyanobacteria.

