Related Experiment Video
Updated: Mar 27, 2026
10:12
Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
Published on: June 12, 2015
9.7K
High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry
Veerendra Kalyan Jagannadh1, Bindu Prabhath Bhat2, Lourdes Albina Nirupa Julius2
1Optics, Microfluidics Instrumentation Lab, Department of Instrumentation & Applied Physics, Indian Institute of Science (IISc), Bangalore, India, 560012.
Analytical and Bioanalytical Chemistry
|January 20, 2016
Summary
This study introduces a cost-effective microfluidic microscopy system for high-throughput cell analysis. The novel instrument achieves rapid blood cell counting at over 125,000 cells per minute, advancing point-of-care diagnostics.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Analytical Instrumentation
Background:
- Microfluidic systems offer miniaturization advantages for biological analyses.
- High-throughput analysis is crucial for rapid diagnostics but often requires expensive equipment.
- Current microfluidic microscopy systems face limitations in throughput.
Purpose of the Study:
- To develop a novel, inexpensive, high-throughput analytical instrument based on microfluidic microscopy.
- To enhance throughput in miniaturized microfluidic microscopy systems.
- To demonstrate the instrument's utility in point-of-care diagnostics through blood cell counting.
Main Methods:
- Development of a miniaturized microfluidic microscopy system with a novel throughput enhancement approach.
- Utilizing cost-effective, low frame rate cameras (120 fps).
- Performing quantitative blood cell counting and comparative analysis.
Main Results:
- Achieved a throughput of approximately 125,880 cells per minute.
- Demonstrated high-throughput analysis using low-cost components.
- Validated the instrument's performance against a commercial hematology analyzer for blood cell counts (cells per μl).
Conclusions:
- The developed microfluidic microscopy system provides an inexpensive yet high-throughput solution for analytical instrumentation.
- The achieved throughput represents a significant advancement for rapid quantitative testing in diagnostics.
- The instrument shows promise for point-of-care diagnostic applications, particularly for blood cell counting.
